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| Mccombie, Helen; Ledu, Christophe; Phelipot, Pascal; Lapegue, Sylvie; Boudry, Pierre; Gerard, Andre. |
| We present a new method to produce tetraploid Crassostrea gigas by cytochalasin B inhibition of polar body 2 expulsion in diploid females crossed with tetraploid males. This offers a means of direct introgression of genetic characters from selected diploid to tetraploid lines, avoiding a triploid step. Offspring larval ploidy shifted over time and depended on size, with tetraploids more frequent among the smaller larvae and triploids among the large. Viable tetraploids were found at 4 and 6 months, indicating the technique was successful. The possibility that gynogenesis occurred was tested by microsatellite analysis to confirm the presence of paternally inherited alleles. These were present in all animals of the 2n x 4n + CB (female first) cross. However,... |
| Tipo: Text |
Palavras-chave: Oyster breeding; Gynogenesis; Polar body; Cytochalasin B; Polyploidy; Crassostrea gigas. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-632.pdf |
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| Ledu, Christophe; Mccombie, Helen. |
| The effects of cytochalasin B on fertilization and ploidy in Crassostrea gigas were examined in a controlled experiment. This enabled us to better understand why variable ploidy results have been found with the retention of polar bodies in previous studies on bivalve mollusks, and what the interaction with fertilization may be. By looking at the early development of Crassostrea gigas embryos with sequential samples stained using Hoechst 33258, a fluorescent DNA-specific dye, we compared normal evolution of fertilized eggs with that obtained with cytochalasin B treatment. In treated eggs, embryos with both three and four pronuclei were found after the second meiotic division. When embryos had three pronuclei, these were identified as the female pronucleus... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Fertilization; Cytochalasin B; Polar body; Polyploidy. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-2845.pdf |
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| Feng,D.Q.; Zhou,Y.; Ling,B.; Gao,T.; Shi,Y.Y.; Wei,H.M.; Tian,Z.G.. |
| Mesenchymal stem cells (MSCs) have been reported to secrete a variety of cytokines and growth factors acting as trophic suppliers, but little is known regarding the effects of conditioned medium (CM) of MSCs isolated from femurs and tibias of mouse on the artificial activation of mouse oocytes and on the developmental competence of the parthenotes. In the current study, we investigated the effect of CM on the events of mouse oocyte activation, namely oscillations of cytosolic calcium concentration ([Ca²+]i), meiosis resumption, pronucleus formation, and parthenogenetic development. The surface markers of MSCs were identified with a fluorescence-activated cell sorter. The dynamic changes of the spindle and formation of pronuclei were examined by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mesenchymal stem cells; Oocyte activation; Parthenogenesis; Calcium; Cytochalasin B. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600006 |
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| Mezzalira,Alceu; Viera,Arnaldo Diniz; Barbieri,Dilmar Paulo; Machado,Mariana Fernandes; Thaler Neto,André; Bernadi,Mari Lourdes; Silva,Carlos Antônio Mondino; Rubin,Mara Iolanda Batistella. |
| In this study, the influence of cytochalasin B on the survival and development after vitrification of oocytes and in vitro produced (IVP) bovine blastocysts was evaluated. In the Experiment I, 956 oocytes were matured for 22h and were immediately vitrified (Vitri treatment) or exposed for 15-20 minutes, to 7.5µg/ml (CB7.5Vitri treatment) or 45µg/ml (CB45Vitri treatment) cytochalasin B solutions, before vitrification. After 30 seconds of exposure to SV1 [400µl TCM-Hepes with 10% fetal serum (SF), 50µl ethylene glycol (EG) and 50µl DMSO], and 20 seconds to SV2 (300µl trehalose 1,0M + 20% SF, 100µl EG and 100µl DMSO) solutions, the oocytes were vitrified in Open Pulled Straws (OPS). The rewarming was performed at 37-38ºC in two steps of 5 minutes each, into... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; Cytochalasin B; Embryos. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000500008 |
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